A Specific and Sensitive Aptamer-Based Digital PCR Chip for Salmonella typhimurium Detection

A Specific and Sensitive Aptamer-Based Digital PCR Chip for Salmonella typhimurium Detection

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Food poisoning and infectious diseases caused by Salmonella typhimurium (S.typhimurium) are serious public health Clothing - Womens Bottoms - Pants concerns for human health and food safety.The diversity and complexity of food matrices pose great challenges for rapid and ultra-sensitive detection of S.typhimurium in food samples.A method capable of identification, detection, and quantification of S.

typhimurium is essential for addressing these issues.In this study, aptamer-coated magnetic beads (Apt-MBs) are employed as capture bio-probes to specifically and selectively concentrate S.typhimurium in food samples.A self-priming chip-based digital PCR was then presented as another biosensor for on-site detection and quantification of S.typhimurium cells.

The chip we developed was robust and did not require any external power for Lamps sample loading.The combination of Apt-MBs with an on-chip digital detection realized the integration into lab-on-a-chip-based biosensors for on-site monitoring of foodborne pathogens.It was possible to capture and detect S.typhimurium cells as low as 90 CFU/reaction with a capture efficiency of 94.5%.

Additionally, the whole process only took about 2 h.This unique platform could also be used to monitor other target bacteria with high specificity and sensitivity by utilizing different aptamers.Furthermore, the platform has potential applications in point-of-care testing in the future.